Highly stable magnetite modified with chitosan, ferrocene and enzyme for application in magneto-switchable bioelectrocatalysis

Journal of the Brazilian Chemical Society

Iost, Rodrigo M.¹  Luz, Roberto A. S.¹  Universidade de São Paulo, São Carlos, Brazil¹  Crespilho, Frank N.¹  Nantes, Iseli L.¹  Universidade Federal do ABC, Santo André, Brazil¹  Melo, Antonio F. A. A.¹ 

关键词: magneto-switchable bioelectrocatalysis;    magnetite;    chitosan;    ferrocene;    glucose oxidase;   

DOI  :  10.5935/0103-5053.20130037

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

Magnetic fields have been used in Bioelectrochemistry to carry enzymes or redox mediators immobilized on magnetite (Fe3O4) to the electrodes surface, providing a switchable control of faradaic current from biocatalysis. In this work, it is reported an advance in the magnetic control of bioelectrochemical reactions, by construction of a system containing simultaneously a magnetic particle (for controlled driving), an enzyme (for biocatalysis) and a redox mediator (for mediation of electron transfer). The advance was attained by synthesis of a new material (Fe3O4-Chi-Fc/GOx) that consists of Fe3O4 particles modified with insoluble ferrocene (Fc) and chitosan (Chi) cross-linked with glucose oxidase (GOx). When this material was used in electrochemical studies, an increase of 70% was observed in the catalytic current of glucose oxidation when 0.24 T was applied perpendicularly to electrode plane. This is the first time that a control of the bioelectrocatalytic process was achieved using enzyme, mediator and magnetite in a unique system switched by a magnetic field.

【 授权许可】

Unknown