Optimization of electrophoretic separations of thirteen phenolic compounds using single peak responses and an interactive computer technique

Journal of the Brazilian Chemical Society

Ballus, Cristiano A.¹  Scarminio, Ieda S.¹  Universidade Estadual de Campinas, Campinas, Brazil¹  Instituto Federal de São Paulo, Campinas, Brazil¹  Bortoloti, João¹  Bruns, Roy E.¹  Faculdade de Engenharia de Alimentos¹  Meinhart, Adriana D.¹  Faculdade Municipal Professor Franco Montoro, Mogi Guaçu, Brazil¹  Godoy, Helena T.¹  Universidade Estadual de Londrina, Londrina, Brazil¹  Câmara, Carlos Alberto P.¹ 

关键词: capillary zone electrophoresis;    central composite design;    phenolic compounds;    response surface optimization;    Derringer-Suich desirability function;   

DOI  :  10.5935/0103-5053.20130219

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

An interactive computer method is proposed for the electrophoretic separation of 13 phenolic compounds from extra-virgin olive oil using single peak response values. A central composite design was executed for optimization of the sodium tetraborate concentration, pH and applied voltage. Statistical models were determined for eight resolution responses and thirteen effective mobilities. Six of the resolution models had highly significant ANOVA lack of fit values, limiting their accuracies for use in Derringer´s desirability function search for optimal separation conditions. None of the 13 effective mobility models suffered from significant lack of fit. Since it is not possible to define effective mobility target values for the desirability function, an interactive computer program developed in our laboratories was applied to the single peak models. Mouse or cursor movements were executed to define experimental conditions in model simulations of the electropherogram. These simulations resulted in superior peak separations, especially for the apigenin and luteolin peaks, in 35 min, compared with those obtained in close to 50 min with the resolution models. Verification experiments performed 2 and 3 years later confirmed the robustness of the models.

【 授权许可】

Unknown