Simultaneous quantification of lycopene, β-carotene, retinol and α -tocopherol in plasma after a simple extraction procedure: stability study and application to human volunteers

Journal of the Brazilian Chemical Society

Bulcão, Rachel P.¹  Garcia, Solange C.¹  Saldiva, Paulo H.¹  Freitas, Fernando¹  Baierle, Marília¹  Bubols, Guilherme B.¹  Durgante, Juliano¹  Moro, Angela M.¹  Brucker, Natália¹  Federal University of Rio Grande do Sul, Porto Alegre, Brazil¹  University of São Paulo, São Paulo, Brazil¹  Federal University of Santa Maria, Santa Maria, Brazil¹  Nascimento, Sabrina¹  Bohrer, Denise¹  Charão, Mariele F.¹ 

关键词: retinol;    α-tocopherol;    lycopene;    β-carotene;    HPLC;    liquid-liquid extraction;   

DOI  :  10.1590/S0103-50532012005000001

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

A method for the simultaneous quantification of lycopene, β-carotene, retinol and α-tocopherol by high-performance liquid chromatography (HPLC) with Vis/fluorescence detection with isocratic elution was optimized and validated. The method consists of a rapid and simple liquid-liquid extraction procedure and a posterior quantification of extracted supernatants by HPLC. Aliquots of plasma were stored at –20°C for three months for stability study. The methodology was applied to samples from painters and individuals not exposed to paints (n = 75). The assay was linear for all vitamins (r > 0.99). Intra- and inter-run precisions were obtained with coefficient of variation smaller than 5%. The accuracies ranged from 0.29 to –5.80% and recoveries between 92.73 and 101.97%. Plasma samples and extracted supernatants were stable for 60 days at –20°C. A significant decrease of lycopene, β-carotene and retinol concentrations in plasma from exposed individuals compared to non-exposed individuals (p < 0.05) was observed. The method is simple, reproducible, precise, accurate and sensitive, and can be routinely utilized in clinical laboratories.

【 授权许可】

Unknown