Ultra-performance liquid chromatographic method for measurement of voriconazole in human plasma and oral fluid

Journal of the Brazilian Chemical Society

Linden, Rafael¹  Boeira, Paula¹  Andreolla, Huander F.¹  Universidade Feevale, Novo Hamburgo, Brazil¹  Pasqualotto, Alessandro C.¹  Antunes, Marina V.¹  Santa Casa de Porto Alegre, Porto Alegre, Brazil¹ 

关键词: voriconazole;    UPLC-PDA;    oral fluid;    drug monitoring;   

DOI  :  10.1590/S0103-50532012000100021

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

A simple, sensitive and selective ultra-performance liquid chromatography method for the determination of voriconazole in plasma and oral fluid was developed and validated. After a liquid-liquid extraction with methyl-tert-butyl ether, the analyte and internal standard were separated on a Hypersil Gold C18 column (2.1 × 100 mm, p.d. 1.9 µm), eluted with a mobile phase composed of thietylammonium phosphate buffer and acetonitrile (70:30, v/v). Total run time was 4 min, total mobile phase consumption of 2.2 mL. Detection was performed with a photodiode array detector with quantitation at 256 nm. Voriconazole concentrations in oral fluid were on average 57.5% (±5.3) of those measured in paired plasma samples.

【 授权许可】

Unknown