A multicommuted flow analysis method for the photometric determination of amoxicillin in pharmaceutical formulations using a diazo coupling reaction

Journal of the Brazilian Chemical Society

Freitas, Sueny K. B¹  Araújo, Alberto N¹  Paim, Ana Paula S¹  Reis, Boaventura F¹  Universidade Federal de Pernambuco, Recife, Brazil¹  Montenegro, Maria Conceição B. S. M¹  Universidade de São Paulo, Piracicaba, Brazil¹  Silva, Valdinete Lins da¹  Universidade do Porto, Porto, Portugal¹ 

关键词: amoxicillin;    multicommuted flow analysis;    photometry;    diazo coupling reaction;    o-nitroaniline;   

DOI  :  10.1590/S0103-50532011000200013

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

A multicommuted flow analysis method for the spectrophotometric determination of amoxicillin in pharmaceutical formulations is reported. The proposed method is based on the reaction of diazotised o-nitroaniline with amoxicillin in an alkaline medium, producing a yellow compound that was monitored at 435 nm. The main features of the method were: a linear relationship with amoxicillin concentration ranging from 25 to 400 mg L-1; a detection limit of 5.1 mg L-1; an analytical throughput of 50 determinations per hour; and a relative standard deviation lower than 3.9% for a typical amoxicillin sample with concentration of 100 mg L-1 (n = 10). To verify the accuracy of the method, samples were also analyzed employing an independent method, and applying the paired t-test to the results no significant difference at the 95% confidence level was observed.

【 授权许可】

Unknown