Attenuated total reflectance with Fourier transform infrared spectroscopy (ATR/FTIR) and different PLS Algorithms for simultaneous determination of clavulanic acid and amoxicillin in powder pharmaceutical formulation

Journal of the Brazilian Chemical Society

Universidade Federal do Pampa, Uruguaiana, Brazi¹  Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil¹  Flores, Érico M. M.¹  Müller, Aline L. H.¹  Müller, Edson I.¹  Instituto Nacional de Ciência e Tecnologia de Bioanalítica, Campinas, Brazil¹  Silva, Fabiana E. B.¹  Ferrão, Marco F.¹  Universidade Federal de Santa Maria, Santa Maria, Brazil¹ 

关键词: clavulanic acid;    amoxicillin;    ATR/FTIR;    pharmaceutical products;    backward interval PLS;   

DOI  :  10.1590/S0103-50532011001000011

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

A method for simultaneous determination of clavulanic acid (CA) and amoxicillin (AMO) was developed using Fourier transform mid infrared technique coupled with attenuated total reflectance (ATR/FTIR) accessory. 27 samples were used as calibration set and 8 samples were used for prediction set. Calibration models were developed using partial least squares (PLS), interval PLS (iPLS), synergy PLS (siPLS) and backward PLS (biPLS). Multiplicative scatter correction and the mean centering were used and produced the best models. Relative standard error of prediction (RSEP) of 3.8% for CA and 5.1% for AMO were obtained using biPLS algorithm for ATR/FTIR data. Results obtained by the proposed methodology were compared with those using high performance liquid chromatography (HPLC) and no significant differences were obtained. The proposed method using ATR/FTIR combined to multivariate analysis methods was suitable for the simultaneous determination of CA and AMO in commercial pharmaceutical products.

【 授权许可】

Unknown