Ultra-performance liquid chromatographic method for simultaneous quantification of HIV non-nucleoside reverse transcriptase inhibitors and protease inhibitors in human plasma

Journal of the Brazilian Chemical Society

Linden, Rafael¹  Sprinz, Eduardo¹  Poeta, Júlia¹  Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil¹  Universidade Feevale, Novo Hamburgo, Brazil¹  Ribeiro, Jorge P.¹  Antunes, Marina V.¹ 

关键词: antiretroviral drugs;    ultra-performance liquid chromatography;    therapeutic drug monitoring;    UPLC-DAD;   

DOI  :  10.1590/S0103-50532011000100018

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

A fast ultra-performance liquid chromatographic with diode-array detection method has been developed and validated for the determination of six protease inhibitors (indinavir, amprenavir, saquinavir, atazanavir, lopinavir, and ritonavir) and two non-nucleoside reverse transcriptase inhibitors (efavirenz and nevirapine). After liquid-liquid extraction of 0.5 mL plasma with methyl-tert-butyl ether, the analytes were separated on a ACQUITY UPLC BEH® C18 column (2.1 × 150 mm, p.d. 1.7 μm) column eluted with a gradient of acetonitrile and triethylammonium phosphate buffer 5 mmol L-1 pH 3.0. The total run time was 9.5 min. Calibration curves were linear in the range 0.1 to to 10.0 μg mL-1. The lower limit of quantitation was 0.1 μg mL-1 for all drugs. Accuracy ranged from 94.9 to 103.5%. Both interday and intraday coefficients of variation were less than 7.7% for all analytes. The extraction yields were greater than 88.2%.

【 授权许可】

Unknown