Unusual 1,6-diphenyl-1,3,5-hexatriene (DPH) spectrophotometric behavior in water/ethanol and water/DMSO mixtures

Journal of the Brazilian Chemical Society

Caetano, Wilker¹  Universidade Estadual de Maringá, Maringá, Brazil¹  Universidade de São Paulo, São Carlos, Brazil¹  Hioka, Noboru¹  Cavalheiro, Carla C. S.¹  Batistela, Vagner R.¹  Gracetto, Augusto C.¹  Santos, Willy Glen¹  Oliveira, Hueder P. M. de¹ 

关键词: diphenylhexatriene;    DPH;    DPH fluorescence;    dynamic quenching;    lifetime;   

DOI  :  10.1590/S0103-50532010000800013

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

The absorption spectra of DPH at fixed concentration do not change with water content in organic solvents. It exhibits monomer bands, such as those obtained in ethanol. The absorption did not change for solutions up to 54 and 46% of water in ethanol and DMSO, respectively, for [DPH] = 5.0 × 10-6 mol L-1 at 30 °C. However, at the same experimental conditions, a gradual sharp decay of the DPH fluorescence is observed. It is proposed that water molecules below these water concentration limits act as quenchers of the excited states of DPH. Stern-Volmer quenching constants by intensities measurements are 7.4 × 10-2 (water/ethanol) and 2.6 × 10-2 L mol-1 (water/DMSO). DPH lifetime measurements in the absence and presence of water resulted in 7.1 × 10-2 L mol-1 in water/ethanol, which pointed out that the process is a dynamic quenching by water molecules. For experiments using DPH as probe, this process can affect data, leading to misunderstanding interpretation.

【 授权许可】

Unknown