Esterase screening using whole cells of Brazilian soil microorganisms

Journal of the Brazilian Chemical Society

Marsaioli, Anita J.¹  Universidade Estadual de Campinas, Campinas, Brazil¹  Mantovani, Simone M.¹  Oliveira, Luciana G. de¹ 

关键词: soil bacteria;    miniaturized screening with fluorescent probes;    esterases;   

DOI  :  10.1590/S0103-50532010000800011

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

A miniaturized enzymatic assay using fluorescent probes to reveal esterase producing microorganisms was optimized and applied to screen 64 soil bacterial strains. The best results were validated using traditional non-fluorogenic assays with acetyl and propanoyl phenylethanol to confirm the miniaturized results. The most active microorganisms belong to the genus Bacillus showing esterase activity and good enantiomeric ratios for the resolution of phenylethanol derivatives (E > 30). Part of the microorganisms are kept in our laboratory in glycerol or freeze-dried and the best microorganisms will be deposited in the CBMAI/CPQBA/Unicamp culture collection.

【 授权许可】

Unknown