On-line identification of minor flavones from sugarcane juice by LC/UV/MS and post-column derivatization

Journal of the Brazilian Chemical Society

Queiroz, Emerson F.¹  Hostettmann, Kurt¹  Université de Genève, Genève, Switzerland¹  Universidade de São Paulo, São Carlos, Brazil¹  Colombo, Renata¹  Ndjoko, Karine¹  Yariwake, Janete H.¹ 

关键词: sugarcane juice;    Saccharum officinarum L. (Poaceae);    flavones;    LC/UV/MS;    UV shift reagents;   

DOI  :  10.1590/S0103-50532009000900003

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

This work describes the on-line characterization of minor flavones from sugarcane (Saccharum officinarum) juice by high-performance liquid chromatography coupled to diode array UV detection and mass spectrometry (LC/UV/MS) using atmospheric pressure chemical ionization-collision-induced dissociation (APCI-CID-MS/MS) and post-column derivatization using UV shift reagents. HPLC-UV analysis with shift reagents provided information about the substitution pattern in the flavonoid skeleton and, combined with MS data, these techniques allowed for the on-line identification of five "garapa" flavones: luteolin-8-C-glucosyl-7-O-glucuronide; tricin-7-O-neohesperoside-4'-O-rhamnoside; tricin-7-O-methylglucuronate-4'-O-rhamnoside; tricin-7-O-methylglucuronide; swertisin, while four other compounds were partially identified as glycosylflavones. Only swertisin (7-O-methylapigenin-6-C-glucoside) was reported previously in sugarcane molasses.

【 授权许可】

Unknown