Study of C- and O-glycosylflavones in sugarcane extracts using liquid chromatography: exact mass measurement mass spectrometry

Journal of the Brazilian Chemical Society

McCullagh, Michael¹  Universidade de São Paulo, São Carlos, Brazil¹  Colombo, Renata¹  Yariwake, Janete H.¹  Waters Corporation, Manchester, UK¹ 

关键词: Saccharum officinarum;    flavonoids;    orthogonal acceleration time-of-flight electrospray ionization mass spectrometry (ESI-oa-ToF MS);   

DOI  :  10.1590/S0103-50532008000300016

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

The flavonoids present in sugarcane (Saccharum officinarum) extracts were analyzed by liquid chromatography – mass spectrometry (LC-MS), and a study of the fragmentation patterns of selected flavonoids was conducted using orthogonal acceleration time-of-flight electrospray ionization mass spectrometry (ESI-oa-ToF MS). Seven C- and O-glycosylflavones were identified in the extracts, namely, schaftoside, isoschaftoside, luteolin-8-C-(rhamnosylglucoside), vitexin, orientin, tricin-7-O-neohesperidoside and tricin-7-O-glucoside. Of these, five were identified in the absence of direct comparison with their respective standards. The described method also permitted the differentiation of the 6-C and 8-C isomeric flavones, schaftoside and isoschaftoside. The combination of fragmentation data and exact mass measurement showed to be complimentary to the HPLC-UV-MS techniques previously utilized for isomers discrimination in sugarcane studies.

【 授权许可】

Unknown