期刊论文详细信息
Der Pharmacia Lettre | |
Analytical method development and validation for the determination of Levetiracetam in pharmaceutical formulations by using RP-HPLC | |
A. Chenthilnathan1  S.Gopalakrishnan1  B. Jeyashree1  A. Ramalakshmi1  | |
DOI : | |
学科分类:药理学 | |
来源: Scholars Research Library | |
【 摘 要 】
A simple, reproducible and efficient reversed phase high performance liquid chromatography (RP-HPLC) method has been developed for estimation of a recently approved anti epileptic drug, Levetiracetam in raw material and its pharmaceutical formulations. Separation was done by using mobile phase consisting of diphasic sodium phosphate buffer and acetonitrile in a ratio of 80:20. The separations were carried out on an Inertsil C18 column (250x 4.6mm; 5μm) at a flow rate of 1.5 ml/min. The injection volume was 10 μl and the peaks were detected at 205nm. The linear dynamic response was found to be in the concentration range of 80 μg -130 μg/ml and coefficient of correlation was found to be 0.9994. The percentage recovery of levetiracetam was found to be 98.37%. The proposed method was found to be simple, fast, accurate, precise and reproducible and could be used for routine quality control analysis of levetiracetam in bulk and pharmaceutical dosage forms.【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912040570739ZK.pdf | 144KB | download |