The Evaluation of Islet Purification Methods That Use Large Bottles to Create a Continuous Density Gradient

Cell Medicine

Kobayashi, Naoya¹  Noguchi, Yasufumi¹  Saitoh, Issei¹  Miyagi-Shiohira, Chika¹  Noguchi, Hirofumi¹  Watanabe, Masami¹  Matsushita, Masayuki¹ 

DOI  :  10.3727/215517916X693131

学科分类：生物科学（综合）

来源: Cognizant Communication Corporation

【 摘 要 】

Islet purification is one of the most important steps of islet isolation for pancreatic islet transplantation. The most common method of islet purification is density gradient centrifugation using a COBE 2991 cell processor. However, this method can damage islets mechanically throughits high shearing force. We recently reported that a new purification method using large plastic bottles effectively achieves a high yield of islets from the porcine pancreas. In the present study, we evaluated the methods of making a continuous density gradient. The gradient was producedwith a gradient maker and two types of candy cane-shaped stainless steel pipes. One method was to use a “bent-tipped” stainless steel pipe and to load from a high-density solution to a low-density solution, uploading the stainless steel pipe. The other method was to use a regularstainless steel pipe and to load from a low-density solution to a high-density solution, leaving the stainless steel pipe in place. There were no significant differences between the two solutions in terms of the islet yield, rate of viability or purity, score, or the stimulation index afterpurification. Furthermore, there were no differences in the attainability or suitability of posttransplantation normoglycemia. Our study shows the equivalency of these two methods of islet purification.

【 授权许可】

Unknown