Airborne molds and mycotoxins in Serpula lacrymans–damaged homes

Developmental Biology

David Garon¹  Alain Bourreau⁵  Veronique Andre¹  Chantal Duhamel²  Didier Pottier¹  Estelle Richard¹  Mathieu Guibert¹  Philippe Verite⁴  Jean–Philippe Rioult¹  Valerie Kientz Bouchart³ 

[1] Université de Caen Basse–Normandie, UR ABTE 4651 – ToxEMAC, Centre F. Baclesse, Avenue Général Harris, F–14076 CAEN Cedex 05, France$$;Laboratoire de Microbiologie, Centre Hospitalier Universitaire, Côte de Nacre, F–14000 CAEN, France$$;Laboratoire Départemental Frank Duncombe, 1 Route Rosel, F–14053 Saint–Contest, France$$;Université de Rouen, UR ABTE 4651 – ToxEMAC, 22 Boulevard Gambetta, F–76183 Rouen Cedex 01, France$$;Bureau d’expertise A. Bourreau, rue du Perron, F–14740 Saint–Manvieu–Norrey, France$$

关键词: Fungal aerosols;    indoor environment;    molds;    mycotoxins;    Serpula lacrymans;   

DOI  :  10.5094/APR.2014.038

学科分类：农业科学（综合）

来源: Dokuz Eylul Universitesi * Department of Environmental Engineering

【 摘 要 】

For some years, the degradation of homes by the dry rot fungus Serpula lacrymans increased. This study described, for the first time, the fungal contamination in homes located in Low–Normandy (France) and damaged by Serpula lacrymans. Wood–decaying fungi, airborne molds, fungal species growing on building materials were investigated by cultural and molecular methods. Mycotoxins in the air were quantified by HPLC–MS/MS and the mutagenicity of fungal aerosols was also evaluated using the Ames test. The results showed that Serpula lacrymans was detected in the air for one third of homes with sometimes the co–occurrence of other ligninolytic basidiomycetes species like Donkioporia expansa. Various molds in the air and on materials (117 and 103 species, respectively) were also identified indicating the complexity of indoor mycoflora. Certain recurrent species like Aspergillus versicolor and Penicillium fellutanum were observed both on building materials and in the air. The presence of cellulolytic molds in fungal aerosols and on building materials could be used as an indicator of home degradation. Airborne culturable fungal levels were measured up to 5.8 x 10⁵ Colony Forming Units (CFU) per cubic meter of the air (CFU/m³) depending on the home. Fungal concentrations also depended on the type of collector (filter or liquid) and were significantly correlated with the median of particles between 2–15 μm in size. Two mycotoxins (alternariol and/or ochratoxin A) were observed in 4 homes but no mutagenic activity was found.

【 授权许可】

Unknown   

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