Molecular evidence confirms presence of anamorph of Erysiphe diffusa on soybean (Glycine max) in northeast India

Australasian Plant Disease Notes

Shishomvanao Ngachan¹  Satish Chandra¹  Pankaj Baiswar¹ 

[1] ICAR Research Complex for NEH Region$$

关键词: E. glycines;   

DOI  :  10.1007/s13314-016-0213-6

学科分类：农业科学（综合）

来源: Springer

【 摘 要 】

Powdery mildew on soybean was observed in October, 2014. Morphological and molecular characterisation was done for ascertaining the identity of the anamorph. Light and scanning electron microscopy revealed it to be a Pseudoidium anamorph. Morphological characters were examined considering the possibility of presence of Erysiphe glycines, E. diffusa and E. pisi. The shape and dimensions of foot cells and conidia, and wrinkling pattern on conidia were the key features for morphological identification of the pathogen. Molecular identification was done using the Internal Transcribed Spacer (ITS) region of ribosomal DNA and the primer sets ITS5 - ITS4 and ITS1 - PM6. Closely related sequences were included in the maximum likelihood analysis. Morphological and molecular identifications delineate this species as E. diffusa. Accurate pathogen identification is important for disease management and current breeding programs in India.

【 授权许可】

Unknown