Phylogenetic analysis of American hop latent virus in Humulus lupulus surveyed in Tasmania and Victoria, Australia

Australasian Plant Disease Notes

Shane E. Hössel¹  Peter A. Cross¹  Simon P. Whittock²  Alison L. Dann¹ 

[1]Biosecurity and Product Integrity Division$$

[2]Hop Products Australia Limited$$

关键词: DAS-ELISA;   

DOI  :  10.1007/s13314-014-0140-3

学科分类：农业科学（综合）

来源: Springer

【 摘 要 】

During July to August 2013 hop (Humulus lupulus) buds growing in Tasmania (n = 280) and Victoria (n = 200) from nine varieties were sampled and tested for the presence of American hop latent virus (AHLV) by enzyme linked immunosorbent assay and reverse transcription polymerase chain reaction using coat protein gene specific primers. Immunological results showed 153/480 bud samples were positive. RT-PCR resulted in an additional 19 positive samples. Coat protein gene sequence analysis showed 98–99 % identity with AHLV isolates from the USA.

【 授权许可】

Unknown