Polymeric Switch on Lysozyme Activity: Role of Hydrophobic and Electrostatic Interactions

The Journal of Pure and Applied Chemistry Research

Dr. Sumon Ganguli¹  Md. Bellal Hossain² 

[1] Lecturer, Dept. of Applied & Environmental Chemistry, university of Chittagong, Bangladesh$$;Department of Nutrition and Food Engineering, Allied Health Science, Daffodil International University, Dhanmondi R/A, Dhaka-1207, Bangladesh$$

关键词: Compost;    water hyacinth;    Eichornia crassipes;    adsorption;    isoterm adsorption;   

DOI  :  10.21776/ub.jpacr.2013.002.03.148

来源: University of Brawijaya

【 摘 要 】

Enzymes have attracted potential applications in both medicine and biotechnology. In our present study, we show a strategy for switching the enzymatic activity of lysozyme by the complex formation with a cationic smart copolymer. PAMA- g -PEG graft copolymers suppressed the enzymatic activity of lysozyme without any conformational change, indicating the formation of complex and covering the active site of lysozyme by copolymers. The addition of polyanion, poly(acrylic acid) (PAAc), recovered the suppressed enzymatic activity of the lysozyme/polymer complex efficiently. These finding suggest that that hydrophobic interaction coupled with electrostatic interactions has a great role for the complexation and decomplexation of the lysozyme/polymer complex. Circular dichroism (CD) spectral analysis indicated that the conformation of the enzymes maintained largely during the course of the complexation.

【 授权许可】

Unknown