Nitrogenase Activity and IAA Production of Indigenous Diazotroph and Its Effect on Rice Seedling Growth

AGRIVITA, Journal of Agricultural Science

Sumadi Sumadi¹  Purwanto Purwanto²  Tualar Simarmata¹  Yuyun Yuwariah¹ 

[1] Faculty of Agriculture Padjadjaran University BandungJl. Raya Jatinangor KM 21 Sumedang, West Java Indonesia 45363$$;Faculty of Agriculture Jenderal Soedirman University PurwokertoJl. Dr. Suparno KP 125 Purwokerto Central Java Indonesia 53122$$

关键词: diazotroph;    IAA;    N fixation;    nitrogenase;    plant growth;   

DOI  :  10.17503/agrivita.v39i1.653

学科分类：农业科学（综合）

来源: Universitas Brawijaya * Fakultas Pertanian / Brawijaya University, Faculty of Agriculture

【 摘 要 】

The diazotroph bacteria as ecofriendly biofer-tilizers play an important role in improving the N status and availability of paddy soil. Laboratory experiment to study nitrogenase activity and IAA production of diazotroph from rice rhizosphere and to assess its effect on the growth of rice seedling has been conducted from September to October 2014 in Agronomy and Horticulture Laboratory Faculty of Agriculture Jenderal Soedirman University, Purwokerto. The experiment was arranged as a completely randomized design and consisted of seven treatments and provided with 4 replications. The treatments were the isolates of indegenous diazotroph (T 1 = A11003, T 2 = A230041, T 3 = A24001, T 4 = A230022, T 5 = A230021, T 6 = A230042 and T 7 = without inoculation). The nitrogenase activity measured by acetylene reduction assay method and IAA production was measured by HPLC method. Plant height, leaf greeness, leaf area, total dry weight and total root length were determined on 21 days after sowing. The experimental results showed that the isolat A230021 was identified as Rhizobium sp. LM-5 and have the highest nitrogenase activity at 0.07 µM C 2 H 4 ml -1 h -1 and IAA production reached 19.01 ppm. Inoculation with strains of diazotroph enhanced chlorophyl content, total root length, and biomass production.

【 授权许可】

Unknown