Human Myocardial mRNA-Expression of Insulin-Dependent Transmembrane Glucose Transporter is Increased in Human IDDM and Decreased in NIDDM

Journal of Clinical and Basic Cardiology

Barth S¹  Gasser R¹  Rigler B¹  Friehs I¹  Mächler H¹  Gasser S¹  Cichocki L¹  Klein W¹ 

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关键词: Glukosetransport;    Hypertonie;    Kardiologie;    cardiology;    gene expression;    glucose transport;    hypertension;   

DOI  :  

学科分类：心脏病和心血管学

来源: Krause & Pachernegg GmbH

【 摘 要 】

Transmembrane glucose transport and thus cellular high energy metabolism of the cardiovascular system depend largely on the insulin responsive GLUT4-isoform of the transmembrane glucose transport molecule. Several authors have shown that, in animals, myocardial GLUT4-mRNA expression is decreased in experimental diabetes. There are no data on humans as yet. Here, we investigate probes of right atrial auricle from diabetic and non diabetic patients subjected to cardiac surgery which were snap frozen in liquid nitrogen. Semiquantitative PCR has been used in order to quantify GLUT4-mRNA using G3PDH as a house keeping gene.Our results represent the first measurements of GLUT4-mRNA in human myocardial tissue. Seven patients had NIDDM (determined byOGT, HbA1C and insulin secretion), 7 had IDDM and 7 served as controls. Relative GLUT4-expression amounted to 97.0 ± 10.4 ( ± SEM)in the control group, 133.3 ± 15.2 in the IDDM-group and 49.1 ± 6.2 ( ± SEM) in the NIDDM-group. From these data we deduce that DMis initially associated with a decreased GLUT4-expression, the latter then being upregulated by external application of insulin.

【 授权许可】

Unknown