The effect of solvent composition on complex formation of S100B protein with peptides

Proceedings of the Estonian Academy of Sciences

Tõnu Kesvatera¹  Heiki Vija¹ 

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关键词: bioorganic chemistry;    S100B;    protein;    calcium sensor;    peptide;    charges;    solvent;   

DOI  :  10.3176/proc.2009.1.12

学科分类：化学（综合）

来源: Teaduste Akadeemia Kirjastus

【 摘 要 】

Complex formation of a vertebrate calcium sensor protein S100B with peptides  TRTKIDWNKILS (A) and SHLKSKKGQSTSRHKKLMWKTE (B) was studied. In contrast with previous reports, the affinity of these peptides for S100B was found to be similar. Added DTT, CaCl2, NaCl, and change in pH differentially reduce the affinity of the two peptides up to two orders of magnitude in the studied range. The effect of added salt is more pronounced for binding peptide B with a higher positive net charge. We conclude that it is necessary to have carefully controlled media conditions in affinity measurements of S100B protein with its targets. This is important in search for efficient S100B blockers of medical interest.

【 授权许可】

Unknown