DNA binding and cleavage activity by a mononuclear iron(II)Schiff base complex: Synthesis and structural characterization

Journal of Chemical Sciences

Soumitra Hazra¹  Balachandran Unni Nair3²  Bhaskar Biswas1³  Subramaniyam Rajalakshmi²  Abhijit Pal³  Merry Mitra³  Chandra Shekhar Purohit⁴  Gopinatha Suresh Kumar¹  Rajarshi Ghosh1³ 

[1] Biophysical Chemistry Laboratory, Chemistry Division, CSIR-Indian Institute of Chemical Biology, Kolkata 700 032, India$$;Chemical Laboratory, CSIR-Central Leather Research Institute, Adayar, Chennai 600 020, India$$;Department of Chemistry, The University of Burdwan, Burdwan 713 104, India$$;Department of Chemical Sciences, National Institute of Science Education and Research, Bhubaneswar 751 005, India$$

关键词: Iron(II);    Schiff base;    X-ray structure;    DNA binding;    DNA cleavage.;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Synthesis and characterization of a mononuclear Fe(II) compound [Fe(L)](ClO4)2 (1) [L = N-(1-pyridin-2-yl-phenylidene)-N'-[2-({2-[(1-pyridin-2-ylphenylidene)amino]ethyl}amino)ethyl] ethane-1,2-diamine] (1) is reported. 1 crystallizes in P-1 space group with a = 11.9241(3) Å, b = 12.1994(3) Å and c = 13.0622(4) Å. The binding property of the complex with DNA has been investigated using absorption and emission studies, thermal melting, viscosity experiments and circular dichroism studies. The binding constant (𝐾b) and the linear Stern-Volmer quenching constant (𝐾sv) of the complex have been determined as 3.5 × 103M-1 and 2.73 × 104M-1, respectively. Spectroscopic and hydrodynamic investigations revealed intercalative mode of binding of 1 with DNA. 1 is also found to induce oxidative cleavage of the supercoiled pUC 18 DNA to its nicked circular form in a concentration dependent manner.

【 授权许可】

Unknown