Antileishmanial polyphenols from Corymbia maculata

Journal of Chemical Sciences

Alka Choudhary³  William J Foley²  Sushma Singh¹  Dinesh Neeradi¹  Inder Pal Singh1³  Jasmeen Sidana1³ 

[1] Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER), Sector-67, SAS Nagar, Punjab 160 062, India$$;Research School of Biology, The Australian National University, Canberra 0200, Australia$$;Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Sector-67, SAS Nagar, Punjab 160 062, India$$

关键词: Corymbia maculata;    flavonoid glycosides;    methylated flavonoids;    myrciaphenone;    HPLC quantitation.;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

An activity-guided fractionation was used to identify the antileishmanial compounds of Corymbia maculata. The hexane, ethyl acetate and methanol extracts were active in in vitro antileishmanial assay. Twelve polyphenols including 8-demethyl eucalyptin (1), eucalyptin (2), myrciaphenone A (3), myrciaphenone B (4), quercetin-3-𝑂-𝛽-D-xylopyranoside (5), myricetin-3-𝑂-𝛼-L-rhamnopyranoside (6), quercetin-3-𝑂-𝛽-D-galactopyranoside (7), quercetin-3-𝑂-𝛽-D-glucopyranoside (8), quercetin-3-𝑂-𝛼-L-rhamnopyranoside (9), syringic acid (10), gallic acid-3-methyl ether (11), gallic acid-4-methyl ether (12) and gallic acid (13) were isolated from the active extracts. All the tested compounds except 8-demethyleucalyptin and myrciaphenone B showed strong to moderate (6.9-24.5 𝜇M) antileishmanial activity against Leishmania donovani promastigotes. An HPLC-PDA method has been developed to detect/quantify 29 compounds in the extracts of C. maculata leaves. This validated method allows simultaneous quantitation of seven flavonoids, fourteen phloroglucinols and eight other polyphenols and can be applied for qualitative as well as quantitative determination of phytoconstituents in Eucalyptus matrices.

【 授权许可】

Unknown