Selective determination of 3,4-dihydroxyphenylacetic acid in the presence of ascorbic and uric acids using polymer film modified electrode

Journal of Chemical Sciences

Palraj Kalimuthu¹  S Abraham John1¹ 

[1]Department of Chemistry, Gandhigram Rural University, Gandhigram 624 302, Dindigul, India$$

关键词: 5-Amino-1;    3;    4-thiadiazole-2-thiol;    3;    4-dihydroxyphenylacetic acid;    ascorbic acid;    uric acid;    amperometry.;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

We report here the highly sensitive and selective electrochemical determination of 3,4-dihydroxyphenylacetic acid (DOPAC), one of the dopamine metabolites in the presence of important interferents ascorbic acid (AA) and uric acid (UA) using an ultrathin electropolymerized film of 5-amino-1,3,4-thiadiazole-2-thiol (p-ATT) modified glassy carbon (GC) electrode in 0.20M phosphate buffer solution (pH 5.0). The bare GC electrode fails to resolve the oxidation peaks of AA, DOPAC and UA in a mixture. Further, the oxidation peak potentials of them were shifted to more positive potential with decreased peak currents in the subsequent cycles. On the other hand, the p-ATT modified electrode not only separated the voltammetric signals of AA, DOPAC and UA but also enhanced their peak currents. The amperometric current response was increased linearly with increasing DOPAC concentration in the range of 4.0 × 10−8 to 1.0 × 10−5 M and the detection limit was found to be 150pM (𝑆𝑁 =3).

【 授权许可】

Unknown