Lasianthera Africana leaves inhibits α-amylase α-glucosidase, angiotensin-I converting enzyme activities and Fe2+-induced oxidative damage in pancreas and kidney homogenates

Oriental Pharmacy and Experimental Medicine

Sunday I. Oyeleye¹  Sidiqat A. Shodehinde²  Adeniyi A. Adebayo³  Tosin A. Olasehinde⁵  Aline A. Boligon⁴  Ganiyu Oboh³ 

[1] Federal University of Technology, Akure$$Federal University of Technology, Akure$$;Adekunle Ajasin University$$;Federal University of Technology, Akure$$;Federal University of Santa Maria$$;Federal Institute of Industrial Research$$

关键词: Angiotensin-1-converting enzyme antioxidant;   

DOI  :  10.1007/s13596-017-0256-z

学科分类：药学

来源: Springer

【 摘 要 】

Lasianthera africana is a dark green leafy vegetable commonly consumed as food and used for treatment of several human ailments in Nigeria most especially south-east. This study investigated the phenolic composition and effects of methanol extract from L. africana leaves on enzymes linked to type-2 diabetes (α-amylase and α-glucosidase) and hypertension [angiotensin-1-converting enzymes (ACE)] as well as Fe²⁺-induced lipid peroxidation in rat’s pancreas and kidney homogenates were evaluated. Phenolic contents and constituents were also determined using colorimetric methods and high performance liquid chromatography-diode array detector (HPLC-DAD) respectively. Our findings revealed that the extract inhibited α-amylase, α-glucosidase and ACE activities with IC₅₀ values of 0.192 and 0.21 mg/mL, and 57.06 μg/mL respectively. The extract also inhibited Fe²⁺-induced lipid peroxidation in rats’ pancreas and kidney homogenates. Our findings revealed that the extract is rich in phenolic acids such as caffeic acid (59.97 mg/g), chlorogenic acid (58.23 mg/g) and ellagic acid (31.16 mg/g) as well as flavonoids such as quercetin (42.98 mg/g). These results give a clue to the potential use of L. africana leaves as an alternative agent for the control of glucose absorption and lowering of blood pressure in diabetic patients with hypertension.

【 授权许可】

Unknown