Identification of aberrant DNA methylation involved in chemoradiation-resistant HCT116 cells via methylation-specific microarray

Molecular & Cellular Toxicology

Jian Dong¹  Yun-Feng Li¹  Ting Chen¹  De-Pei Gao¹  Guan-Shun Wang¹  Ying-Ying Ding¹  Shan Liu¹  Wei Xiong¹  Hong-Tao Zhang¹  Zhi-Bin Yang¹ 

[1] The Third Affiliated Hospital of Kunming Medical University$$

关键词: HCT116 cells;   

DOI  :  10.1007/s13273-016-0039-5

学科分类：分子生物学，细胞生物学和基因

来源: Korean Society of Toxicogenomics and Toxicoproteomics

【 摘 要 】

Preoperative 5-fluorouracil (5-FU) based chemoradiation is regarded as the standard treatment for locally advanced colorectal cancer (CRC). However, advances in using 5-FU in chemoradiotherapy treatment of CRC are limited by the resistant to the chemoradiotherapy. It has been demonstrated that aberrant methylation contributes to radioresistance in a human colorectal tumor cell line. So we analyzed the differential DNA methylation patterns by methylationspecific microarray in a 5-FU-based concurrent chemoradiotherapy resistance cell model which was established by using human CRC cell line HCT116. As a result, we detected a total of 3719 hypomethylated sites and 27940 hypermethylated sites in CRR cells compared to the control. Furthermore, we analyzed top 10 GO terms involved in significantly differential DNA methylation patterns resulted from the 5-FU-based CRR through GO analyses. To further explore the molecular basis of 5-FU-based CRR in CRC cells, CYP1B1 and CYP2C9 genes expression level were shown down regulated by qRT-PCR. These findings might provide a novel insight for chemoradiation resistance in CRC and provide a potential therapy method in CRC patients.

【 授权许可】

Unknown