Effect of freezing stress on the proteome expression of Antarctic green microalga

Molecular & Cellular Toxicology

Mi-Young Lee¹  Kwang-Man Choi¹ 

[1] SoonChunHyang University$$

关键词: Antarctic green microalga;   

DOI  :  10.1007/s13273-012-0020-x

学科分类：分子生物学，细胞生物学和基因

来源: Korean Society of Toxicogenomics and Toxicoproteomics

【 摘 要 】

The effects of freezing stress on the protein expression profile and enzyme activity change in Antarctic green microalga were examined in this investigation. Antarctic green microalga was cultured at 4°C for optimal growth, and the temperature was reduced near to 0°C in order to apply freezing stress. The resultant differentially expressed proteins induced by freezing stress were analyzed by mass spectrometry following two-dimensional gel electrophoresis (2-DE). In total, about 320 protein spots were detected on the 2-DE gel, and 17 proteins showed a greater than two-fold change in abundance; of these, 5 proteins were up-regulated, and 12 proteins were down-regulated. Moreover, total enzyme activities and isozyme patterns of several antioxidant enzymes, named catalase, superoxide dismutase and aldehyde dehydrogenase were changed upon applying freezing stress.

【 授权许可】

Unknown