A Relation Between Cell Cycle and Intestinal Metaplasia in Oesophageal Biopsies Using Optical and Digital Microscopy

Pathology & Oncology Research

Béla Molnár¹  Miklós Máté² 

[1] Semmelweis University$$;Saint Emerich Hospital Budapest$$

关键词: Optical and digital microscopy;   

DOI  :  10.1007/s12253-014-9873-8

学科分类：生理学与病理学

来源: Springer

【 摘 要 】

Protein expression changes in relation to cell cycles provide important information, and it may represent a new method for an early diagnosis of metaplasia �? dysplasia �? adenocarcinoma sequence. We investigated potential changes in cell cycle genes such as protooncogenes (PCNA, EGFR), tumour suppressor gene (p53), apoptotic TUNNEL (Tdt mediated dUTP nick and labelling) gene, as well as small intestinal mucus antigen (SIMA) and large intestinal mucus antigen (LIMA), which accumulates in metaplastic epithelium due to the inflammatory process in routine oesophageal biopsies using immunohistochemistry. Oesophageal biopsies were taken from patients with Barrett’s oesophagus (n�?=�?30), reflux oesophagitis (n�?=�?30), healthy oesophagus (n�?=�?30) and healthy cardia (n�?=�?10). Immunohistochemical signalling was carried out by Streptavidin-Biotin-AEC (aminoetil-carbazol). Expression of PCNA was statistically significantly lower in healthy oesophagus (p�?<�?0.05) versus reflux oesophagitis and Barrett’s oesophagus. However, no significant change was detected in the expression of SIMA and LIMA in intestinal metaplasia. Further, EGFR, p53 and TUNNEL levels were significantly different in healthy versus Barrett’s oesophagus. Manual counting using virtual microscopy was comparable with the result using conventional light microscopy, but the former is significantly quicker. There was no difference between manual and automated cell counting (p�?>�?0.05).

【 授权许可】

Unknown