Classical and Alternative Nuclear Factor-κB Pathways: A Comparison among Normal Prostate, Benign Prostate Hyperplasia and Prostate Cancer

Pathology & Oncology Research

Fu-neng Jiang¹  Yu-xiang Liang¹  Wei-de Zhong³  Yan-ru Chen¹  Hui-chan He¹  Jian-guo Zhu²  Guo-hua Zeng⁴  Jia-hong Chen¹  Qi-shan Dai¹  Chao Cai¹  Zhao-dong Han¹  Guo-qiang Qin¹  Xi-bin Chen¹ 

[1] Guangzhou First Municipal People’s Hospital, Affiliated Guangzhou Medical College$$;Guizhou Provincial People’s Hospital$$;Guangzhou First Municipal People’s Hospital, Affiliated Guangzhou Medical College$$The First Affiliated Hospital of Guangzhou Medical College, Guangzhou Medical College$$Affiliated Guangzhou Medical College$$;The First Affiliated Hospital of Guangzhou Medical College, Guangzhou Medical College$$

关键词: Real-time quantitative RT-PCR;   

DOI  :  10.1007/s12253-011-9396-5

学科分类：生理学与病理学

来源: Springer

【 摘 要 】

Nuclear factor-κB (NF-κB) is controlled by the classical and alternative NF-κB pathways, the role of which in prostate cancer (PCa) is not clearly defined. To provide this missing translational link, we compared the classical and alternative NF-κB pathways in normal prostate, benign prostate hyperplasia (BPH) and PCa. Prostate specimens were divided into three groups: group A, PCa (n�?=�?68); group B, BPH (n�?=�?60); and group C, normal prostates (n�?=�?15). The gene expression levels of NF-κB1 and NF-κB2 were determined by real-time quantitative RT-PCR. Additionally, we analyzed the expression and sub-cellular localization of phosphorylated P50 (p-P50) and phosphorylated P52 (p-P52) proteins by immunohistochemical staining. Furthermore, associations were made between NF-κB pathway proteins and patients�? prognosis. Compared with BPH and normal prostate tissues, the expression of NF-κB1 gene was differentially down-regulated by >1.5-fold, whereas NF-κB2 gene was differentially up-regulated by >2-fold in PCa tissues. The proportion of p-P50 positive patients in group A (26.5%) was significantly lower than in group B (88.3%, p�?=�?0.005) and C (100%, p�?=�?0.002). The proportion of p-P52 positive patients in group A (42.6%) was significantly higher than in group B (11.7%, p�?=�?0.009) and C (6.7%, p�?=�?0.008). Comparison of the survival curves in group A according to p-P52 expression showed a significant difference between positive and negative patients. The p-P52 positive patients showed worse prognosis (p�?=�?0.019). Our findings suggest for the first time that the classical and alternative NF-κB pathways have an important role in PCa. p-P52 might be a predictor of poor prognosis for PCa.

【 授权许可】

Unknown