Differential regulation of umbilical cord blood and leukemic B cells by interferon-alpha (IFN-α): Observations in cultured cells

Pathology & Oncology Research

Csongor Kiss²  János Szöllösi⁵  Éva Karászi⁴  György Vámosi³  Péter Kovács¹  Ilona Benkő¹  István Szegedi² 

[1] University of Debrecen$$;Department of Pediatrics$$;Department of Cell Biophysics Research Group of the Hungarian Academy of Sciences$$;Department of Clinical Biochemistry and Molecular Pathology$$;Department of Cell Biophysics Research Group of the Hungarian Academy of Sciences$$Department of Biophysics and Cell Biology$$

关键词: type II Fcγ receptor;   

DOI  :  10.1007/BF02893363

学科分类：生理学与病理学

来源: Springer

【 摘 要 】

The exact mechanism of the beneficial therapeutic action of interferon-a (IFN-α) in B-cell-lineage malignancies has not been adequately explained. Here we report on the differential effect of IFN-α2b on non-malignant B cells of umbilical cord blood and leukemic B-cell lines JY, BL-41 and BCBL-1. Leukemic cell proliferation was characterized by colony assay, whereas apoptosis was investigated by flow cytometry of propidium iodide-stained cells. The degree of differentiation was evaluated by measuring the expression level of Fcγ receptor-II (FcγRII) labeled with anti-CD32-FITC monoclonal antibody using flow cytometry. IFN-α protected umbilical cord blood CD19-positive B lymphocytes from apoptotic cell death in vitro. IFN-α significantly decreased colony formation of all three cell lines, and in contrast to normal cells, induced apoptosis in JY and BL-41 and excessive necrosis in HHV-8 infected BCBL-1 cells. FcγRII was upregulated both in normal and in leukemic B cells as indicated by an increase both in the proportion of CD32-positive cells and the mean fluorescence intensity. From our results it seems that antiproliferative, apoptotic and differentiative effects of IFN-a are interrelated but distinct cellular events, which are differentially regulated in normal, leukemic and virus-infected cells of the B-cell lineage. (Pathology Oncology Research Vol 12, No 3, 159�?163)

【 授权许可】

Unknown