Tumor-infiltrating B cell immunoglobulin variable region gene usage in invasive ductal breast carcinoma

Pathology & Oncology Research

Beatrix Kotlan³  József Tóth¹  David I. Stott²  Jean-Luc Teillaud⁴  Peter Simsa³ 

[1] National Institute of Oncology$$;University of Glasgow, Western Infirmary$$;Institute of Haematology and Immunology$$;Centre de Recherches Biomedicales des Cordeliers$$

关键词: tumor-infiltrating lymphocytes;   

DOI  :  10.1007/BF02893374

学科分类：生理学与病理学

来源: Springer

【 摘 要 】

A major focus of tumor immunology is to reveal the potential role and capacity of immunocompetent cells found in different solid tumor tissues. The most abundant infiltrating cells (TIL), the T lymphocytes have been investigated in details concerning T-cell receptor usage and specificity. However, B cells have hardly been investigated in this respect, although high cellular B-cell infiltration has been correlated with improved patients�? survival in some breast carcinomas. This led to our objectives to study variable region gene usage of the tumor-infiltrating B cells in different breast carcinoma types. By defining the immunoglobulin repertoire of the tumor-infiltrating B lymphocytes in the most common invasive ductal carcinoma (IDC) of the breast we compared it to the rare medullary breast carcinoma (MBC). After phenotyping infiltrating ductal carcinomas, B cells were obtained from tumor tissue by microdissection technique. Numerous rearranged TIL-B immunoglobulin heavy chain V genes (VH) were amplified, cloned, sequenced, and comparatively analyzed. Some characteristics were found for both breast carcinoma types. The immunoglobulins produced by TIL-B in ductal carcinoma are highly matured and oligoclonal. We conclude that Ig variable region gene usage reveals similar and distinguishable characteristics of TIL-B immunoglobulin repertoires, which are representative of the nature of the immune responses in invasive ductal and medullary breast carcinomas.

【 授权许可】

Unknown