p53 protein accumulation and p53 gene mutation in colorectal cancer

Pathology & Oncology Research

Jerzy Ostrowski¹  Anna Nasierowska-Guttmejer²  Lech Trzeciak³  Marek P. Nowacki² 

[1] Medical Center for Postgraduate Education$$Maria Skodowska-Curie Memorial Cancer Center and Institute of Oncology$$;Maria Skodowska-Curie Memorial Cancer Center and Institute of Oncology$$;Medical Center for Postgraduate Education$$

关键词: colorectal cancer;   

DOI  :  10.1007/BF03187331

学科分类：生理学与病理学

来源: Springer

【 摘 要 】

Comparison of immunohistochemical methods for detection of protein p53 accumulation and molecular techniques for analysis p53 gene mutation in colorectal cancer is presented. Thirty eight patients were included: all underwent surgery without preoperative treatment. Sex of patients, tumor localisation, macro and microscopic type of cancer and staging according to Astler-Coller and Jass classifications were evaluated. Protein p53 accumulation was detected by the streptavidin-biotin method using DO-7 (Dako) antibody. The number of cells stained were classified semiquanititatively according to a scoring system: (�?) no positive cells, (+) : 10�?30% positive cells, (++) : 40�?70% positive cells, (+++) : >70% positive cells. For all cancer samples, exons 5 to 9 of p53 gene were amplified from isolated genomic DNA. PCR products were subjected to single standed conformational polymorphism analysis. All product were also directly sequenced on ABI PRISM 377 apparatus using fluorescent dideoxyterminators chemistry. The protein p53 accumulation was detected in 53% (20/38), whereas p53 gene mutation was seen in 55% (21/38). Among them, 15 patients (39%) with overexpression showed mutation in exon 5�?8 gene p53. Discrepancies between results were noted in 29%. In conclusion, the necessity of both methods �? immunohistochemical and molecular �? is indicated for the objective evaluation of functional and structural status of p53 gene and protein.

【 授权许可】

Unknown