Pericytes display increased CCN2 expression upon culturing

Journal of Cell Communication and Signaling

Andrew Leask²  David J. Abraham¹  Vineeth Rajkumar¹  Xu Shiwen¹  Christopher P. Denton¹ 

[1] University College London (Royal Free Campus)$$;University of Western Ontario$$

关键词: CCN2;   

DOI  :  10.1007/s12079-009-0053-7

学科分类：分子生物学，细胞生物学和基因

来源: Springer

【 摘 要 】

By providing a source of α-smooth muscle actin (α-SMA)-expressing myofibroblasts, microvascular pericytes contribute to the matrix remodeling that occurs during tissue repair. However, the extent to which pericytes may contribute to the fibroblast phenotype post-repair is unknown. In this report, we test whether pericytes isolated from human placenta can in principle become fibroblast-like. Pericytes were cultured in vitro for 11 passages. The Affymetrix mRNA expression profile of passage 2 and passage 11 pericytes was compared. The expression of type I collagen, thrombospondin and fibronectin mRNAs was induced by passaging pericytes in culture. This induction of a fibroblast phenotype was paralleled by induction of connective tissue growth factor (CTGF/CCN2) and type I collagen protein expression and the fibroblast marker ASO2. These results indicate that, in principle, pericytes have the capacity to become fibroblast-like and that pericytes may contribute to the population of fibroblasts in a healed wound.

【 授权许可】

Unknown