Effects of lead chloride on human erythrocyte membranes and on kinetic anion sulphate and glutathione concentrations

Cellular & Molecular Biology Letters

Grazia Luca¹  Caterina Rigano¹  Adriana Scuteri¹  Leonardo Romano¹  Pietro Romano¹  Tiziana Gugliotta¹ 

[1] Dipartimento di Scienze della Vita “M. Malpighi”, Facoltà di Scienze MM.FF.NN., Università degli Studi di Messina, Messina, Italy$$

关键词: Anion transport;    Band 3 protein;    Erythrocytes;    Erythrocyte membrane;    GSH;    GSSG;    Lead chloride;    Scanning electron microscopy;    Anemia;    Adenosine 5′ triphosphate;    Sulphate influx;   

DOI  :  10.2478/s11658-012-0027-2

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

Our study concerns the effects of exposure to lead chloride on the morphology, K+ efflux, SO4 − influx and GSH levels of the human erythrocyte. Blood was collected in heparinized tubes and washed three times. The cells were suspended at 3% hematocrit and incubated for 1 h at 25°C in a medium containing increasing concentrations of lead chloride (0, 0.3, 0.5 and 1 μM). After incubation, the suspensions were centrifuged and the erythrocyte pellets were divided into three aliquots for testing. The results show: an increase in the permeability of erythrocytes treated with lead chloride with consequent damage and cellular death, especially in the presence of high concentrations; an increase in potassium ion efflux; alterations in the morphology and membrane structure of the red blood cells; and a decrease in sulphate uptake, due either to the oxidative effect of this compound on the band 3 protein, which loses its biological valence as a carrier of sulphate ions, or to a decrease in the ATP erythrocyte concentration. In conclusion, the exposure of erythrocytes to Pb2+ ions leads to a reduction in the average lifetime of the erythrocytes and the subsequent development of anemia. These data are discussed in terms of the possible effect of lead on the reduction-oxidation systems of the cell. Oxidant agents, such as lead, are known to cross-link integral membrane proteins, leading to K/Cl-cotransport. The increased K+ efflux affects the altered redox state.

【 授权许可】

Unknown