Protective effect of placenta extracts against nitrite-induced oxidative stress in human erythrocytes

Cellular & Molecular Biology Letters

Svitlana Rozanova¹  Oleg Nardid¹  Svitlana Repina¹  Yana Cherkashina¹  Katherina Rozanova¹ 

[1] Department of Cryobiophysics, Institute for Problems of Cryobiology and Cryomedicine, NAS of Ukraine, Kharkiv, Ukraine$$

关键词: Human placenta extract;    Protein;    Antioxidant activity;    Oxidative stress;    Nitrite;    Erythrocyte;    Methemoglobin;    Cytosol;    Freezing;    Thawing;   

DOI  :  10.2478/s11658-012-0007-6

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

Aqueous-saline human placenta extract (HPE) is known to possess antioxidant activity due to the high concentration of bioactive substances. This fact allows its application in clinical practice in order to treat oxidation-induced diseases. Extract antioxidant activity is mainly conditioned by proteins. Freezing of extracts has been shown to lead to their antioxidant activity increasing due to protein conformation changes. Different biological models are widely used in order to evaluate efficacy of novel antioxidants and mechanisms of their action. One such model appears to be erythrocytes under nitrite-induced oxidative stress. Nitrite is known to be able to penetrate erythrocyte membrane and to oxidize hemoglobin. In order to investigate whether HPE is able to decrease nitrite-induced oxidative injuries and to evaluate the protein contribution to this process, spectrophotometric and electron spin resonance (ESR) assays were used. Experimental data have revealed that antioxidant activity of extracts and of some of their fractions correlates with methemoglobin concentration lowering. Preliminary erythrocyte incubation with an extract fraction of 12 kDa allows preservation of the structural-dynamic cytosol state the closest to the control.

【 授权许可】

Unknown