Maintenance of rat hepatocytes under inflammation by coculture with human orbital fat-derived stem cells

Cellular & Molecular Biology Letters

Xia Chen¹  Yingjie Wang²  Shichang Zhang²  Tao Liu²  Yong Liu² 

[1] Department of Infectious Disease, Southwest Hospital Affiliated to Third Military Medical University, Chongqing, China$$Department of Pediatrics, the 324th hospital of PLA, Chongqing, China$$;Department of Infectious Disease, Southwest Hospital Affiliated to Third Military Medical University, Chongqing, China$$

关键词: Acute liver failure;    Coculture;    Differentiation;    Fat tissue;    Hepatocytes;    Inflammation;    Interleukin-6;    Mesenchymal stem cells;    Orbital fat-derived stem cells;    Serum;   

DOI  :  10.2478/s11658-012-0004-9

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

Preservation of hepatocyte functions in vitro will undoubtedly help the management of acute liver failure. The coculture system may be able to prevent functional decline of hepatocytes. It has already been shown that hepatocytes, when cocultured with bone marrow mesenchymal stem cells, could undergo long-term culture in vitro without loss of functions. In this study, human orbital fat-derived stem cells were isolated and cocultured with rat hepatocytes. When treated with serum from an acute liver failure patient, rat hepatocyte monoculture showed reduction of cell viability and loss of liverspecific functions. However, rat hepatocytes in the coculture system were still able to secret albumin and synthesize urea. IL-6 was significantly elevated in the coculture of rat hepatocyte with orbital fat-derived stem cells, and it might be the key immunoregulator which protects rat hepatocytes against inflammation. Our data confirmed that orbital fat-derived stem cells, or other adipose tissue-derived stem cells, are an ideal candidate to support rat hepatocyte functions in vitro.

【 授权许可】

Unknown