Rho kinase inhibitors stimulate the migration of human cultured osteoblastic cells by regulating actomyosin activity

Cellular & Molecular Biology Letters

Xuejiao Zhang¹  Huiling Gao¹  Hiroyuki Wakisaka¹  Hiroaki Nabeka¹  Naoto Kobayashi²  Seiji Matsuda¹  Cheng Li¹  Tetsuya Shimokawa¹ 

[1] Department of Anatomy and Embryology, Ehime University Graduate School of Medicine, Ehime, Japan$$;Medical Education Center, Ehime University School of Medicine, Shitsukawa, To-on City, Ehime, Japan$$

关键词: Osteoblast;    Migration;    ROCK inhibitor;    Cytoskeleton;    Stress fiber;    Focal contact;    MLC phosphorylation;   

DOI  :  10.2478/s11658-011-0006-z

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

We investigated the effects of Rho-associated kinase (ROCK) on migration and cytoskeletal organization in primary human osteoblasts and Saos-2 human osteosarcoma cells. Both cell types were exposed to two different ROCK inhibitors, Y-27632 and HA-1077. In the improved motility assay used in the present study, Y-27632 and HA-1077 significantly increased the migration of both osteoblasts and osteosarcoma cells on plastic in a dose-dependent and reversible manner. Fluorescent images showed that cells of both types cultured with Y-27632 or HA-1077 exhibited a stellate appearance, with poor assembly of stress fibers and focal contacts. Western blotting showed that ROCK inhibitors reduced myosin light chain (MLC) phosphorylation within 5 min without affecting overall myosin light-chain protein levels. Inhibition of ROCK activity is thought to enhance the migration of human osteoblasts through reorganization of the actin cytoskeleton and regulation of myosin activity. ROCK inhibitors may be potentially useful as anabolic agents to enhance the biocompatibility of bone and joint prostheses.

【 授权许可】

Unknown