The age-dependent induction of apoptosis-inducing factor (AIF) in the human semitendinosus skeletal muscle

Cellular & Molecular Biology Letters

Ha Young Kim²  Kyoung Ho Yoon³  Seong Kyu Park²  Jung Hwan Lee¹  Mun Seog Chang²  Soo Yeon Park¹ 

[1]Sports Medicine Center, East-West Neo Medicial Center, Kyung Hee University, Seoul, Korea$$

[2]Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University, Seoul, Korea$$

[3]Department of Orthopaedic Surgery, School of Medicine, Kyung Hee University, Seoul, Korea$$

关键词: Human skeletal muscle;    Semitendinosus;    Apoptosis;    Aging;    Apoptosis-inducing factor;   

DOI  :  10.2478/s11658-009-0030-4

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

To assess the dependence on age of the expression of apoptosis regulatory proteins in the human semitendinosus muscle, we measured the expression levels of several apoptosis-related genes, including apoptosis-inducing factor (AIF), Bax, Bcl-2, caspase-3 and heat shock protein 70 (HSP70), using RT-PCR, immunohistochemistry and TUNEL assays. We found that the DNA fragmentation was proportional to the age of the tissues sample donors. The expression levels of AIF were significantly elevated (by 10 to 25%) in semitendinosus tissue samples from older individuals, but the Bax, Bcl-2, caspase-3 and HSP 70 levels remained almost constant. This data suggests that the morphological and functional changes observed in aged human semitendinosus muscle correlates with the apoptosis of muscle cells through the induction of AIF.

【 授权许可】

Unknown