The intrabody targeting of hTERT attenuates the immortality of cancer cells

Cellular & Molecular Biology Letters

Shenghua Liang¹  Daming Ren¹  Guimei Yang¹  Xiangying Zhu¹  Nan Yang¹  Jianguo Cai¹ 

[1]State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Fudan, P. R. China$$

关键词: Cancer;    Intrabody;    Anti-hTERT ScFv;    Immortality;   

DOI  :  10.2478/s11658-009-0032-2

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

hTERT (human telomerase reverse transcriptase) plays a key role in the process of cell immortalization. Overexpression of hTERT has been implicated in 85% of malignant tumors and offers a specific target for cancer therapy. In this paper, we describe an effective approach using a single-chain variable fragment (scFv) intrabody derived from monoclonal hybridoma directed against hTERT to attenuate the immortalization of human uterine cervix and hepatoma cells. The scFv we constructed had a high affinity to hTERT, and specifically neutralized over 70% of telomere synthesis activity, thereby inhibiting the viability and proliferation of the cancer cells. Our results indicate that this anti-hTERT intrabody is a promising tool to target hTERT and intervene in the immortalization process of cancer cells.

【 授权许可】

Unknown