The effects of disodium pamidronate on human polymorphonuclear leukocytes and platelets: An in vitro study

Cellular & Molecular Biology Letters

Arianna Vignini²  Laura Mazzanti²  Eleonora Salvolini¹  Monia Orciani¹  Roberto Primio¹ 

[1] Department of Molecular Pathology and Innovative Therapies - Histology, Polytechnic University of Marche, Ancona, Italy$$;Institute of Biochemistry, Polytechnic University of Marche, Ancona, Italy$$

关键词: Pamidronate;    Polymorphonuclear leukocytes;    Platelets;    Nitric oxide;    Intracellular calcium;    Myeloperoxidase activity;   

DOI  :  10.2478/s11658-009-0012-6

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

Recent reports have indicated that, as well as having antiresorptive effects, bisphosphonates could have an application as anti-inflammatory drugs. Our aim was to investigate whether this anti-inflammatory action could be mediated by the nitric oxide (NO) released by the leukocytes migrating to the site of inflammation. In particular, we investigated in vitro the intracellular calcium concentration ([Ca2+]i), the level of NO released by PMN and platelets, and the PMN myeloperoxidase activity after incubation with disodium pamidronate, since there was a postulated modulatory effect of this aminosubstituted bisphosphonate on leukocytes both in vitro and in vivo. Our data shows that the pamidronate treatment provoked a significant increase in the [Ca2+]i parallel to the enhancement in NO release, suggesting a possible activation of constitutive nitric oxide synthase, while the myeloperoxidase activity was significantly reduced. In conclusion, we hypothesized that treatment with pamidronate could stimulate NO-production by cells present near the bone compartment, thus constituting a protective mechanism against bone resorption occurring during inflammation. In addition, PMN- and platelet-derived NO could act as a negative feed-back signal to restrict the inflammatory processes.

【 授权许可】

Unknown