The effects of superoxide dismutase knockout on the oxidative stress parameters and survival of mouse erythrocyt

Cellular & Molecular Biology Letters

Błażej Rychlik⁴  Ewa Macierzyńska⁴  Łukasz Pułaski¹  Marcin Kruszewski²  Łukasz Piotrowski⁴  Agnieszka Grzelak⁴  Grzegorz Bartosz³ 

[1] Department of Molecular Biophysics, University of Łódź, Łódź, Poland$$Laboratory of Transcriptional Regulation, Centre of Medical Biology, Polish Academy of Sciences, Łódź, Poland$$;Department of Molecular Biophysics, University of Łódź, Łódź, Poland$$Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Warsaw, Poland$$;Department of Molecular Biophysics, University of Łódź, Łódź, Poland$$Department of Biochemistry and Cell Biology, University of Rzeszów, Rzeszów, Poland$$;Department of Molecular Biophysics, University of Łódź, Łódź, Poland$$

关键词: Superoxide dismutase;    Erythrocyte;    Red blood cell;    Reactive oxygen species;    Oxidative stress;    Heinz bodies;    Acetylcholinesterase;   

DOI  :  10.2478/s11658-008-0031-8

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

The erythrocytes of 12-month old Sod1 −/− mice showed an increased level of reactive oxygen species (ROS), as estimated by the degree of dihydroethidine and dihydrorhodamine oxidation, and the increased level of Heinz bodies. No indices of severe oxidative stress were found in the red blood cells and blood plasma of Sod1 −/− mice as judged from the lack of significant changes in the levels of erythrocyte and plasma glutathione, plasma protein thiol and carbonyl groups and thiobarbituric-acid reactive substances in the blood plasma. However, a decreased erythrocyte lifespan, increased reticulocyte count and splenomegaly were noted, indicating the importance of superoxide dismutase for maintaining erythrocyte viability. The levels of erythrocyte ROS and Heinz bodies and the reticulocyte count were indistinguishable in Sod1 +/+ and Sod1 +/− mice, suggesting that a superoxide dismutase activity decrease to half of its normal value may be sufficient to secure the protective effects of the enzyme.

【 授权许可】

Unknown