Engrafting fetal liver cells into multiple tissues of healthy adult mice without the use of immunosuppressants

Cellular & Molecular Biology Letters

Kristina Wilhelm⁴  Renata Gasparaviciute³  Jurij A. Kozhevnikov¹  Adas Darinskas²  Evaldas Liutkevicius⁵  Ludmilla A. Morozova-Roche⁴  Audrone Pilinkiene⁵  Mantas Malisauskas⁴ 

[1] Laboratory of Cell Biotechnology, the Sibirian Division of the Russian Academy of Sciences, Institute of Clinical Immunology, Novosibirsk, Russia$$;Laboratory of Immunopharmacology, Institute of Immunology, Vilnius University, Vilnius, Lithuania$$;Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius, Lithuania$$;Department of Medical Biochemistry and Biophysics, Umeå University, Umeå, Sweden$$;“Imunolita” JSC, Vilnius, Lithuania$$

关键词: Fetal cells;    Transplantation;    Engraftment;    Immune suppression;   

DOI  :  10.2478/s11658-007-0013-2

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

We have shown the fetal liver cell engraftments into multiple tissues of adult healthy mice, achieved without suppressing the animals’ immune systems. Fetal cells from the livers of male C57Bl/6J Black lineage mice at day 13 to 15 of gestation were injected intravenously into female adult CC57W/MY White mice. The grafting was evaluated by Y-chromosome-specific PCR, cytometric analysis of fluorescently stained donor cells, and histological analysis. All the methods consistently showed the presence of multiple engraftments randomly distributed through the various organs of the recipients. After 60 days, the grafts still constituted 0.1 to 2.75% of the tissues. The grafted cells did not change their appearance in any of the organs except the brain, where they became enlarged. Inflammatory reactions were not detected in any of the histological preparations. The frequency of engraftments was higher in the liver, indicating that similarity between the donor and recipient cells facilitates engraftment. The high inherent plasticity of fetal liver cells underlies their ability to integrate into healthy recipient organs, which can be governed by environmental conditions and connections with neighboring cells rather than by the initial cellular developmental programs. The fact that fetal liver cells can be grafted into multiple tissues of healthy animals indicates that they can be used to replace the natural loss of cells in adult organisms.

【 授权许可】

Unknown