Genetic diversity of SIRE-1 retroelements in annual and perennial glycine species revealed using SSAP

Cellular & Molecular Biology Letters

Stephen R. Pearce¹  Amar Kumar²  Catherine Chesnay² 

[1] School of Life-Sciences, University of Sussex, Brighton, UK$$;Scottish Crop Research Institute, Invergowrie, Dundee, UK$$

关键词: Retroelement;    SSAP;    phylogeny;    SIRE-1;   

DOI  :  10.2478/s11658-006-0054-y

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

Sequence Specific Amplification Polymorphisms (SSAP) were used to measure the distribution and structure of SIRE-1 retroelement populations in annual and perennial Glycine species. For SSAP analysis, primers corresponding to a region immediately upstream of the 3’LTR of the soybean retroelement SIRE-1 were chosen. Analysis reveals that SIRE-1 is present throughout the Glycine genus and shows that the annual species have similar SIRE-1 populations whilst the perennial species have much more distinct and diverse populations. The high number of species-specific subgroups suggest that SIRE-1 has been active and evolving independently in each species during the course of Glycine evolution.

【 授权许可】

Unknown