The DefH9-iaaM -containing construct efficiently induces parthenocarpy in cucumber

Cellular & Molecular Biology Letters

Agnieszka Ziółkowska¹  Hans Sommer³  Stefan Malepszy¹  Zhimin Yin²  Robert Malinowski¹  Wojciech Plcader¹ 

[1] Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture and Landscape Architecture, Warsaw Agriculture University, Warsaw, Poland$$;Institute of Plant Genetics, Polish Academy of Science, Poznań, Poland$$;Department of Molecular Plant Genetics, Max Planck Institut für Zuechtungsforschung, Cologne, Germany$$

关键词: Fruit set;    Cucumber;    Ovary-specific promoter;    Transgenic parthenocarpy;   

DOI  :  10.2478/s11658-006-0024-4

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

Parthenocarpy (seedless fruits) is a desirable trait that has been achieved in many plant cultivars. We generated parthenocarpic cucumber fruits by introducing the chimeric DefH9-iaaM construct into the cucumber genome using an Agrobacterium tumefaciens-mediated protocol. The construct consists of the DefH9 promoter from Antirrhinum majus and the iaaM coding sequence from Pseudomonas syringae. Transgenic plants were obtained from nine independent transformation events: half of these were tetraploid and did not produce seeds following self-pollination, while the remaining half were capable of displaying parthenocarpy in the subsequent reproductive generation. Of the fruits produced by the transgenic lines, 70–90% were parthenocarpic. The segregation of the marker gene in the transgenic T1 progeny indicated single gene inheritance. The seed set in the transgenic lines and their F1 hybrids was lower than in the non-transgenic control plants. Some of the methodological details and the practical significance of the results are discussed.

【 授权许可】

Unknown