The transcript expression profile of the leptin receptor-coding gene assayed with the oligonucleotide microarray technique — Could this be an Anorexia nervosa marker?

Cellular & Molecular Biology Letters

Irena Krupka-Matuszczyk²  Joanna Głogowska-Ligus¹  Urszula Mazurek¹  Tadeusz Wilczok¹  Małgorzata Stachowicz¹  Małgorzata Janas-Kozik³ 

[1] Department of Molecular Biology and Medical Genetics, Medical University of Silesia, Katowice, Poland$$;Department and Clinic of Psychiatry and Psychotherapy, Medical University of Silesia, Katowice, Poland$$;Department and Clinic of Psychiatry and Psychotherapy, Medical University of Silesia, Katowice, Poland$$Department of Child and Adolescent Psychiatry, Medical University of Silesia, Katowice, Poland$$

关键词: Anorexia nervosa;    Leptin receptor;    Oligonucleotide microarray;   

DOI  :  10.2478/s11658-006-0006-6

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

Anorexia nervosa is a serious eating disorder with the highest mortality rate of any psychiatric disorder. The DSM-IV classification differentiates two AN types: the restricting type (AN-R) and the binge-eating/purging type (AN-BP). Leptin (LEP) levels can be thought of as a signal to the body of its energy reserves. The leptin receptor (including all its mRNA isoforms) is expressed in many tissues. Our aim was to discover the transcript expression profile of the LEP receptor-coding gene in the peripheral blood mononuclears in AN-R and AN-BP patients. Three young women suffering from Anorexia nervosa (one with AN-BP and two with AN-R) took part in the study, along with three non-anorexic subjects as our reference group. LEP receptor gene expression was examined using the oligonucleotide microarray method (HG-U133A, Affymetrix). The results were normalized using RMAExpress. Next, the accumulation analysis method was used (clustering). Hierarchical clustering resulted in three groups of separate clusters. The first group (cluster I) consisted of AN-R patients. The next group (cluster II) consisted of reference group patients suffering from different psychic disorders not related to eating disorders. Cluster III consisted of two patients — the first with AN-BP and the second with an adaptive disorder.

【 授权许可】

Unknown