Tumor Necrosis Factor-Alpha and 8-Hydroxy-2'-Deoxyguanosine are Associated with Elevated Urinary Angiopoietin-2 Level in Type 2 Diabetic Patients with Albuminuria

Kidney and Blood Pressure Research

Wang Y.-M.²  Su H.²  Wang Q.-Y.²  Jiang H.-J.²  Chen S.²  Huang Q.-X.³  Zhang C.²  He F.-F.²  Li H.-Q.¹ 

[1] Department of Nephrology; $$;Department of Gastrointestinal Surgery, Yantai Yuhuangding Hospital, Yantai, Shandong, China$$

关键词: Angiopoietin 2;    Tumor necrosis factor-alpha;    8-Hydroxy-2'-deoxyguanosine;    Interleukin-18;    Superoxide dismutase;   

DOI  :  10.1159/000368510

来源: S Karger AG

【 摘 要 】

Background/Aims: We previously showed that urine and serum Angiopoietin-2 (Ang-2) levels increased significantly with the degree of albuminuria in diabetes patients, but the reasons remain unclear. Consequently we aimed to determine whether there was an association between Ang-2, inflammatory cytokines (TNF-α and IL-18) and reactive oxygen species (8-OHdG and SOD) in type 2 diabetes patients with albuminuria. Methods: This retrospective study evaluated 113 patients with type 2 diabetes and normoalbuminuria, microalbuminuria, or macroalbuminuria and 30 healthy controls. Serum and urine TNF-α, IL-18 and 8-OHdG levels were measured by ELISA. Superoxide dismutase (SOD) activity was determined by spectrophotometry. Results: Serum and urine TNF-α, IL-18 and 8-OHdG levels increased significantly with the degree of albuminuria, and were positively correlated with increased Ang-2. In contrast, SOD activity decreased with the degree of albuminuria and was negatively correlated with Ang-2. Multivariable linear regression analysis revealed that serum Ang-2 level was independently associated with serum levels of TNF-α (P<0.001), 8-OHdG (P=0.001), and IL-18 (P=0.003). Urinary Ang-2 level was independently associated with urinary TNF-α (P<0.001) and 8-OHdG (P=0.004) levels. Conclusion: TNF-α and 8-OHdG are associated with elevated urinary Angiopoietin-2 levels in type 2 diabetic patients with albuminuria.

【 授权许可】

Unknown