Investigating the Effect of CoCl2 Administration on Diabetic Nephropathy and Associated Aortic Dysfunction

Kidney and Blood Pressure Research

Patel J.K.³  Mishra B.¹  Deshmukh A.B.²  Prajapati A.R.⁴ 

[1] Department of Pharmacology, Shankersinh Vaghela Bapu Institute of Pharmacy, Unava, $$;Pharmaceutics and $$;Department of Quality Assurance, Shree Sarvajanik Pharmacy College, Mehsana, India$$

关键词: Chronic hypoxia;    Hypoxia inducible factor-1α;    Prolyl hydroxylase;    Oxidative stress;    Nitric oxide;    Angiotensin II;    Endothelial dysfunction;   

DOI  :  10.1159/000343888

来源: S Karger AG

【 摘 要 】

Aim: Endothelial dysfunction appears to be a consistent finding in diabetic nephropathy. The study aimed to investigate the effect of cobalt chloride in the amelioration of endothelial dysfunction in uninephrectomized diabetic rats. Methods: We examined the effect of CoCl₂ (10 mg/kg, i.p., OD = once a day) treatment on contractile responses to angiotensin II (10^–10 to 10^–6M) in an aortic preparation of control rats and uninephrectomized diabetic control rats. Blood glucose, plasma urea, creatinine, uric acid, aortic endothelial nitric oxide synthase (eNOS), nitrate/nitrite (NOx), superoxide dismutase, catalase and reduced glutathione levels were checked in the different groups. Results: A significant attenuation of the augmented responses to angiotensin II was observed in CoCl₂-treated animals along with a fall in plasma urea, creatinine and uric acid levels. A significant reduction in blood glucose and an increase in aortic eNOS and NOx levels along with antioxidants levels were observed. Conclusion: Chronic hypoxia augments angiotensin II responses in the thoracic aorta of uninephrectomized diabetic control rats. CoCl₂ attenuates these enhanced vascular responses with a significant decrease in blood glucose signifying stabilization of the hypoxia-inducible factor in the alleviation of endothelial dysfunction in diabetic nephropathy.

【 授权许可】

Unknown