Recent excitements in protein NMR: Large proteins and biologically relevant dynamics

Journal of biosciences

MANDAR V DESHMUKH1¹  SAI CHAITANYA CHILIVERI¹ 

[1] CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500007, India$$

关键词: Biomolecular NMR;    CEST;    CPMG;    DEST;    methyl TROSY;    PRE;    RDCs;    relaxation dispersion;    TROSY;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

The advent of Transverse Relaxation Optimized SpectroscopY (TROSY) and perdeuteration allowed biomolecularNMR spectroscopists to overcome the size limitation barrier (~20 kDa) in de novo structure determination of proteins.The utility of these techniques was immediately demonstrated on large proteins and protein complexes (e.g. GroELGroES,ClpP protease, Hsp90-p53, 20S proteasome, etc.). Further, recent methodological developments such asResidual Dipolar Couplings and Paramagnetic Relaxation Enhancement allowed accurate measurement of long-rangestructural restraints. Additionally, Carr-Purcell-Meiboom-Gill (CPMG), rotating frame relaxation experiments (R1ρ)and saturation transfer experiments (CEST and DEST) created never-before accessibility to the μs–ms timescaledynamic parameters that led to the deeper understanding of biological processes. Meanwhile, the excitement in thefield continued with a series of developments in the fast data acquisition methods allowing rapid structural studies onless stable proteins. This review aims to discuss important developments in the field of biomolecular NMRspectroscopy in the recent past, i.e., in the post TROSY era. These developments not only gave access to the structuralstudies of large protein assemblies, but also revolutionized tools in the arsenal of today’s biomolecular NMR and pointto a bright future of biomolecular NMR spectroscopy.

【 授权许可】

Unknown