Ayurvedic Amalaki Rasayana promotes improved stress tolerance and thus has anti-aging effects in Drosophila melanogaster

Journal of biosciences

SUBHASH C LAKHOTIA1¹  VIBHA DWIVEDI¹ 

[1] Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221 005, India$$

关键词: Aging;    Ayurveda;    cell stress response;    Hsp27;    Hsp70;    Hsp83;    life span;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Amalaki Rasayana (AR) is a common Ayurvedic herbal formulation of Phyllanthus emblica fruits and some otheringredients, and is used for general good health and healthy aging. We reported it to improve life history traits and tosuppress neurodegeneration as well as induced apoptosis in Drosophila. The present study examines responses ofDrosophila reared on AR-supplemented food to crowding, thermal or oxidative stresses. Wild-type larvae/flies rearedon AR-supplemented food survived the various cell stresses much better than those reared on control food. AR-fedmutant park13 or DJ-1βDelta93 (Parkinson’s disease model) larvae/flies, however, showed only partial or no protection,respectively, against paraquat-induced oxidative stress, indicating essentiality of DJ-1β for AR-mediated oxidativestress tolerance. AR feeding reduced the accumulation of reactive oxygen species (ROS) and lipid peroxidation evenin aged (35-day-old) wild-type flies while enhancing superoxide dismutase (SOD) activity. We show that while Hsp70or Hsp83 expression under normal or stress conditions was not affected by AR feeding, Hsp27 levels were elevated inAR-fed wild-type control as well as heat-shocked larvae. Therefore, besides the known anti-oxidant activity ofPhyllanthus emblica fruits, dietary AR also enhances cellular levels of Hsp27. Our in vivo study on a model organismshows that AR feeding significantly improves tolerance to a variety of cell stresses through reduced ROS and lipidperoxidation on the one hand, and enhanced SOD activity and Hsp27 on the other. The resulting better homeostasisimproves life span and quality of organism’s life.

【 授权许可】

Unknown