Quaternary ammonium salt N-(dodecyloxycarboxymethyl)-N,N,N-trimethyl ammonium chloride induced alterations in Saccharomyces cerevisiae physiology

Journal of biosciences

AGATA PIECUCH¹  EWA MACIASZCZYK-DZIUBIŃSKA¹  EWA OBŁĄK1¹  DONATA WAWRZYCKA¹ 

[1] Institute of Genetics and Microbiology, and Institute of Experimental Biology University of WrocÅ‚aw, WrocÅ‚aw, Poland$$

关键词: Drug resistance;    plasma membrane;    quaternary ammonium salts;    iae;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

We investigated the influence of the quaternary ammonium salt (QAS) called IM (N-(dodecyloxycarboxymethyl)-N,N,N-trimethyl ammonium chloride) on yeast cells of the parental strain and the IM-resistant mutant (EO25 IMR)growth. The phenotype of this mutant was pleiotropic. The IMR mutant exhibited resistance to ethanol, osmotic shockand oxidative stress, as well as increased sensitivity to UV. Moreover, it was noted that mutant EO25 appears to havean increased resistance to clotrimazole, ketoconazole, fluconazole, nystatin and cycloheximide. It also toleratedgrowth in the presence of crystal violet, DTT and metals (selenium, tin, arsenic). It was shown that the presence ofIM decreased ergosterol level in mutant plasma membrane and increased its unsaturation. These results indicatechanges in the cell lipid composition. Western blot analysis showed the induction of Pma1 level by IM. RT-PCRrevealed an increased PMA1 expression after IM treatment.

【 授权许可】

Unknown