Diazinon degradation by a novel strain Ralstonia sp. DI-3 and X-ray crystal structure determination of the metabolite of diazinon

Journal of biosciences

GUANGLI WANG¹  YUAN LIU1¹ 

[1] College of Life Sciences, Huaibei Normal University, Huaibei 235000, P.R of China$$

关键词: Biodegradation;    diazinon;    single-crystal X-ray diffraction analysis;    strain DI-3;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Diazinon is a widely used organophosphorus insecticide often detected in the environment. A highly effectivediazinon-degrading Ralstonia sp. strain DI-3 was isolated from agricultural soil. Strain DI-3 can utilize dimethoateas its sole carbon source for growth and degrade an initial concentration of 100 mg·L^{−1} diazinon to non-detectablelevels within 60 h in liquid culture. A small amount of second carbon source as co-substrate could slightly enhance thebiodegradation of diazinon. In addition, a less toxic metabolic intermediate formed during the degradation of diazinonmediated by strain DI-3 was purified using thin-layer chromatography (TLC) and identified based on single-crystal Xraydiffraction analysis, allowing a degradation pathway for diazinon by pure culture to be proposed. Finally, this isthe first providing authentic evidence to describe the metabolite.

【 授权许可】

Unknown